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Table 4 Expression of various pstS promoter fragment cat fusions in C. glutamicum WT

From: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Promoter fragment in transcriptional fusion Carbon source sp. act. of chloramphenicol acetyltransferase [μmol min−1 mg−1]a
   0 min 90 minb
R0F0 Glucose 0.02 2.10
  Acetate 0.01 0.22
RcFc Glucose <0.005 0.61
  Acetate <0.005 0.10
RcFm Glucose <0.005 0.12
  Acetate <0.005 0.01
RmFc Glucose <0.005 0.10
  Acetate <0.005 0.01
RmFm Glucose <0.005 0.02
  Acetate <0.005 <0.005
  1. aAt least three determinations of two independent cultivations were performed. Average values are given with experimental imprecision < 20 %
  2. bThe specific activity of chloramphenicol acetyltransferase was measured 0 and 90 min after a shift from Pi sufficient to Pi limiting conditions