Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

BMC Microbiology

Table 4 Expression of various pstS promoter fragment cat fusions in C. glutamicum WT

Promoter fragment in transcriptional fusion	Carbon source	sp. act. of chloramphenicol acetyltransferase [μmol min⁻¹ mg⁻¹]^a
		0 min	90 min^b
R0F0	Glucose	0.02	2.10
	Acetate	0.01	0.22
RcFc	Glucose	<0.005	0.61
	Acetate	<0.005	0.10
RcFm	Glucose	<0.005	0.12
	Acetate	<0.005	0.01
RmFc	Glucose	<0.005	0.10
	Acetate	<0.005	0.01
RmFm	Glucose	<0.005	0.02
	Acetate	<0.005	<0.005

^aAt least three determinations of two independent cultivations were performed. Average values are given with experimental imprecision < 20 %
^bThe specific activity of chloramphenicol acetyltransferase was measured 0 and 90 min after a shift from P_i sufficient to P_i limiting conditions

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