Table 3 Oligonucleotides used in this study
Oligonucleotide Sequence (5’ → 3’) | |
|---|---|
pstsRforward | CCCCTCGAGTAAAAAAGAGACTTGCTAAAAACCT (XhoI) |
pstsR0forward | CCCCTCGAGTAAGAATCGGTGATTTTCGTTCC (XhoI) |
pstsR1forward | CCCCTCGAGAGAGTCTCCAAATGTTACGAGTGAA (XhoI) |
pstsR2forward | CCCCTCGAGCCTGAGTTAGTCATTTCAAGGTCTTA (XhoI) |
pstsR3forward | CCCCTCGAGGCCCGCCTACAGGATCTGCTCA (XhoI) |
pstsF0reverse | CGTCTAGATGCGGACTGCTGGGAAGATG (XbaI) |
pstsF1reverse | CGTCTAGACCTCAATGGATGCAGCATCGGAAG (XbaI) |
pstsF2reverse | CGTCTAGATCAGACTCATTGGAGTCGGAGCAA (XbaI) |
pstsF3reverse | CGTCTAGAGTTCACGGGGAAGCCTTTCCGG (XbaI) |
pstsF4reverse | CGTCTAGATAAGACCTTGAAATGACTAACTCAGG (XbaI) |
pstsFc_reverse | CGGTTTCCCTCCGGATTGCTCACGACTTAAAAACCTA |
pstsFm_reverse | CGGTTTCCCTCCGGATTGCGCGCGGAGTAAAAACCTA |
pstsRc_forward | CCCGATGTGGGTAGTGGCAGAATTTGCCGAACGAT |
pstsRm_forward | CCCGATGTGGGTAGTGGCAGAAGAGGCCGAACGAT |
pstsF0biotin | Biotin-TGCGGACTGCTGGGAAGATGCAC |