Fig. 5

Binding of RamB to various pstS promoter fragments. RamB protein (0, 150 fold molar excess) was incubated with the full-length pstS promoter fragment (RF0, 5 nM, 507-bp) or the partial length pstS promoter fragments (RmFm, RcFm, RmFc, RcFc, 61 nM, 124 bp) and applied for native polyacrylamide gel electrophoresis. A 267 bp fragment of R3F0, which lacked both of RamB binding sites, served as a negative control