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Fig. 4 | BMC Microbiology

Fig. 4

From: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Fig. 4

Binding of RamB to various pstS promoter fragments. (a) Gel shift assay with RamB and the fragment of the pstS promoter lacking 5' region. RamB protein (0, 100 fold molar excess) was incubated with the full-length pstS promoter (RF0, 507 bp, 15 nM) or the different fragments of the pstS promoter lacking 5' region (R0F0, R1F0, R2F0, R3F0, final concentrations 61 nM – 15 nM) and applied for native polyacrylamide gel electrophoresis. A 185 bp promoter fragment of cg0527 served as a negative control. (b) Gel shift assay with RamB and the fragment of the pstS promoter lacking 3' region. RamB protein (0, 100, 200, 400-fold molar excess) was incubated with the pstS promoter (RF0, 507 bp, 15 nM) or fragment of the pstS promoter lacking 3' region (RF4, 230 bp, 33 nM) and applied for native polyacrylamide gel electrophoresis. A 122 bp promoter fragment of dtxR served as a negative control

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