Identification of RNAIII-binding proteins in Staphylococcus aureus using tethered RNAs and streptavidin aptamers based pull-down assay

Table 2 Bacteria strains and plasmids used in this study

Strain or plasmid	Comments	Source or Reference
Strains
S. aureus NCTC8325	Wild type	NARSA^a
E. coli DH5α	Clone host strain	Laboratory stock
E. coli BL21 (DE3)	Expression host strain	Laboratory stock
E. coli M15 (pREP4)	Expression host strain	Laboratory stock
Plasmids
pcDNA3-tRSA	Template for tRSA	[19]
pEASY TB	Clone vector, Kan^r Ap^r	Transgen
pSXZ06	pEASY TB with RNAIII for in vitro transcription	[36]
pET28a (+)	Expression vector with hexahistidine tag, Kan^r	Novagen
pET21b (+)	Expression vector with hexahistidine tag, Ap^r	Novagen
pQE30	Expression vector with hexahistidine tag, Ap^r	QIAGEN
pQE30-RNase III	Expression vector for protein RNase III	[36]
pET28a-Hu	Expression vector for protein Hu	This study
pET21b-FtsZ	Expression vector for protein FtsZ	This study
pET28a-Pyk	Expression vector for protein Pyk	This study
pET28a-Era	Expression vector for protein Era	This study
pET28a-WalR	Expression vector for protein WalR	[37]
pET22b-RNase J2	Expression vector for protein RNase J2	This study
pET22b-CshA	Expression vector for protein CshA	This study
pET22b-PNPase	Expression vector for protein PNPase	This study
pET22b-PfkA	Expression vector for protein PfkA	This study
pET24a-Enolase	Expression vector for protein Enolase	This study

ISSN: 1471-2180