Skip to main content

Advertisement

Table 2 Bacteria strains and plasmids used in this study

From: Identification of RNAIII-binding proteins in Staphylococcus aureus using tethered RNAs and streptavidin aptamers based pull-down assay

Strain or plasmid Comments Source or Reference
Strains   
S. aureus NCTC8325 Wild type NARSAa
E. coli DH5α Clone host strain Laboratory stock
E. coli BL21 (DE3) Expression host strain Laboratory stock
E. coli M15 (pREP4) Expression host strain Laboratory stock
Plasmids   
pcDNA3-tRSA Template for tRSA [19]
pEASY TB Clone vector, Kanr Apr Transgen
pSXZ06 pEASY TB with RNAIII for in vitro transcription [36]
pET28a (+) Expression vector with hexahistidine tag, Kanr Novagen
pET21b (+) Expression vector with hexahistidine tag, Apr Novagen
pQE30 Expression vector with hexahistidine tag, Apr QIAGEN
pQE30-RNase III Expression vector for protein RNase III [36]
pET28a-Hu Expression vector for protein Hu This study
pET21b-FtsZ Expression vector for protein FtsZ This study
pET28a-Pyk Expression vector for protein Pyk This study
pET28a-Era Expression vector for protein Era This study
pET28a-WalR Expression vector for protein WalR [37]
pET22b-RNase J2 Expression vector for protein RNase J2 This study
pET22b-CshA Expression vector for protein CshA This study
pET22b-PNPase Expression vector for protein PNPase This study
pET22b-PfkA Expression vector for protein PfkA This study
pET24a-Enolase Expression vector for protein Enolase This study
  1. a NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus