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Fig. 3 | BMC Microbiology

Fig. 3

From: Identification of RNAIII-binding proteins in Staphylococcus aureus using tethered RNAs and streptavidin aptamers based pull-down assay

Fig. 3

Expression and EMSA of the binding ability of the protein candidates. a Protein candidates expressed in E. coli. The clone and expression details are listed in Tables 2, 3 and 4. b, c, and d The binding ability of proteins to RNAIII was determined by EMSA. DIG-labeled RNAIII probes (0.24 μM) were used in all reactions. Increasing amounts of different proteins were incubated with labeled RNAIII probes. Figure (b) represents four proteins containing RNA-binding motifs (RNase III 0, 2, 4, 8 μM; CshA 0, 6.5, 32 μM; RNase J2 0, 3.2, 6.4 μM; Era 0, 2.9, 5.8 μM.), Figure (c) represents two proteins containing DNA-binding motifs, and three proteins unrelated to nucleic acid (Hu 0, 180, 360 μM; WalR 0, 75, 150 μM; Pyk 0, 6.3, 13 μM; FtsZ 0, 27, 140 nM; pfkA 0, 16, 140 μM). Figure (d) represents two negative control proteins (PNPase 0, 1.3, 12 μM; Enolase 0, 2.1, 19 μM)

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