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Table 3 Identification of the dominant Campylobacter jejuni strains in the in vivo chicken colonization competition assays by PCR detection of relevant genes

From: Extensive characterization of Campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization

Group and assay

Sample

Sample type

ars C

vir B11

cj0144

tet O

rlo B

cje0256

Group1- assay1

Bird1

bird feces

+

-

+

+

+

-

Group1- assay1

Bird2

bird feces

+

-

+

+

+

-

Group1- assay1

Bird3

bird feces

+

-

+

+

+

-

Group1- assay2

Bird1

bird feces

+

-

+

+

+

-

Group1- assay2

Bird2

bird feces

+

-

+

+

+

-

Group1- assay2

Bird3

bird feces

+

-

+

+

+

-

Group1- assay1 + 2

G2008b

inoculated strain DNA

+

-

+

+

+

-

Group1- assay1 + 2

B2008c

inoculated strain DNA

-

+

-

-

-

-

Group2- assay1

Bird1

bird feces

+

-

+

-

+

-

Group2- assay1

Bird2

bird feces

+

-

+

-

+

-

Group2- assay1

Bird3

bird feces

+

-

+

-

+

-

Group2- assay2

Bird1

bird feces

+

-

+

-

+

-

Group2- assay2

Bird2

bird feces

+

-

+

-

+

-

Group2- assay2

Bird3

bird feces

+

-

+

-

+

-

Group2- assay1 + 2

A2008a

inoculated strain DNA

+

-

+

-

+

-

Group2- assay1 + 2

B2008b

inoculated strain DNA

-

+

-

-

-

-

Group3- assay1

Bird1

bird feces

-

-

+

+

-

-

Group3- assay1

Bird2

bird feces

-

-

+

+

-

-

Group3- assay1

Bird3

bird feces

-

-

+

+

-

-

Group3- assay2

Bird1

bird feces

+ (low)

-

+

+

-

-

Group3- assay2

Bird2

bird feces

+ (low)

-

+

+

-

-

Group3- assay2

Bird3

bird feces

-

-

+

+

-

-

Group3- assay1 + 2

F2008d

inoculated strain DNA

-

-

+

+

-

-

Group3- assay1 + 2

D2008a

inoculated strain DNA

-

-

-

+

-

+

  1. Detection of genes specific to the inoculated strains in the in vivo competition assays seven days post-inoculation; (+) gene detected in the sample; (−) gene not detected in the sample; (low) weak signal observed.