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Table 3 Bacterial viability determination from untreated and heat-fixed kidney tissue obtained from a tissue mimetic model

From: Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging

Bacterial isolate Treatment 1:10 1:100 1:1000 cfu/ml
E.coli Top10 none TNTC 1000+ 1000+ >103
E.coli pGSV3-GmR none TNTC 1000+ 1000+ >103
E.coli pAM17-KanR none TNTC 1000+ 1000+ >103
Klebsiella pneumoniae none TNTC 1000+ 1000+ >103
Burkholderia thailandiensis none TNTC 1000+ 1000+ >103
Acinetobacter baumani none TNTC 1000+ 1000+ >103
Control (no bacteria) none 0 0 0 0
E.coli Top10 heat fixed 0 0 0 0
E.coli pGSV3-GmR heat fixed 0 0 0 0
E.coli pAM17-KanR heat fixed 0 0 0 0
Klebsiella pneumoniae heat fixed 0 0 0 0
Burkholderia thailandiensis heat fixed 0 0 0 0
Acinetobacter baumani heat fixed 0 0 0 0
Control (no bacteria) heat fixed 0 0 0 0
  1. TNTC = too numerous to count, HF = Heat fixed.