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Table 2 Viable bacteria determination from untreated and heat-fixed Burkholderia infected tissue

From: Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging

Pathogen

Tissue

Weight (mg)

Treatment

cfu for

Average cfu/ml

10 −1

B. thailandensis

lung

40

none

1000+

>1 × 105

52

none

1000+

>1 × 105

40

none

1000+

>1 × 105

56

HF

0

0.00

48

HF

0

0.00

34

HF

0

0.00

spleen

61

none

70

7.0 × 104

64

none

71

7.1 × 104

71

none

76

7.6 × 104

57

HF

0

0.00

45

HF

0

0.00

65

HF

0

0.00

B. mallei

lung

ND

none

1000+

>1 × 105

ND

none

1000+

>1 × 105

ND

none

1000+

>1 × 105

ND

HF

0

0.00

ND

HF

0

0.00

ND

HF

0

0.00

  1. HF = Heat fixed, ND=not determined.