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Table 2 Viable bacteria determination from untreated and heat-fixed Burkholderia infected tissue

From: Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging

Pathogen Tissue Weight (mg) Treatment cfu for Average cfu/ml
10 −1
B. thailandensis lung 40 none 1000+ >1 × 105
52 none 1000+ >1 × 105
40 none 1000+ >1 × 105
56 HF 0 0.00
48 HF 0 0.00
34 HF 0 0.00
spleen 61 none 70 7.0 × 104
64 none 71 7.1 × 104
71 none 76 7.6 × 104
57 HF 0 0.00
45 HF 0 0.00
65 HF 0 0.00
B. mallei lung ND none 1000+ >1 × 105
ND none 1000+ >1 × 105
ND none 1000+ >1 × 105
ND HF 0 0.00
ND HF 0 0.00
ND HF 0 0.00
  1. HF = Heat fixed, ND=not determined.