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Table 1 Viable virus determination from untreated and heat fixed VEEV (vaccine strain and virulent strain) infected tissue

From: Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging

Pathogen Tissue Weight (mg) Treatment Dilution and plaque count Average titer (pfu/ml)
10 −1 10 −2 10 −3 10 −4 10 −5 10 −6
VEE (TC83) vaccine strain brain 100 none 100+ 21 3 0 0 0 2.5E + 04
120 none 100+ 30 8 0 0 0 5.5E + 04
140 HF 0 0 0 0 0 0 0.0E + 00
125 HF 0 0 0 0 0 0 0.0E + 00
spleen 47 none 0 0 0 0 0 0 0.0E + 00
52 none 0 0 0 0 0 0 0.0E + 00
55 HF 0 0 0 0 0 0 0.0E + 00
50 HF 0 0 0 0 0 0 0.0E + 00
VEE (Trinidad donkey) virulent strain brain 210 none TNTC TNTC TNTC TNTC TNTC 42 4.2E + 08
240 none TNTC TNTC TNTC TNTC 58 14 9.9E + 07
220 none TNTC TNTC TNTC TNTC TNTC 37 3.7E + 08
190 none TNTC TNTC TNTC TNTC TNTC 34 3.4E + 08
190 HF 0 0 0 0 0 0 0.0E + 00
180 HF 0 0 0 0 0 0 0.0E + 00
260 HF 0 0 0 0 0 0 0.0E + 00
210 HF 0 0 0 0 0 0 0.0E + 00
spleen 60 none TNTC TNTC 42 7 0 0 2.5E + 06
50 none 45 13 0 0 0 0 8.8E + 03
50 none 39 11 0 0 0 0 7.5E + 03
70 none 38 9 0 0 0 0 6.5E + 03
50 HF 0 0 0 0 0 0 0.0E + 00
60 HF 0 0 0 0 0 0 0.0E + 00
50 HF 0 0 0 0 0 0 0.0E + 00
70 HF 0 0 0 0 0 0 0.0E + 00
  1. TNTC = too numerous to count, HF = Heat fixed.