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Table 6 Expression of selected genes in strain Δfst1 relative to expression in wild type (WT) of F. verticillioides

From: Transcriptome changes in Fusarium verticillioides caused by mutation in the transporter-like gene FST1

Gene Name

FVEG ID a

Relative Expression b

Autoclaved Kernels

Living Kernels

FUM1

00316

−33.3 (−25.8, − 48.5)

 

FUM21

14633

−9.4 (−7.6, − 11.7)

 

POD1

10866

−3.9 (−3.7, − 4.1)

−2.0 (−2.0, − 2.0)

POD3

12884

−86.7 (−73.3, − 102.6)

−102.1 (−71.0, −147.0)

POD4

12465

−119.1 (−115.8, − 122.5)

−4.1 (−4.1, − 4.2)

CAT1

05529

−12.0 (−10.1, − 14.2)

−32.4 (−21.8, − 47.1)

CAT2

12611

3.7 (3.3, 4.0)

2.2 (1.9, 2.4)

CAT3

11955

1.5 (1.5, 1.6)

4.9 (4.8, 4.9)

HYD3

06538

−76.0 (−73.3, − 78.7)

 

HYD7

09843

33.7 (31.8, 35.7)

 

ITR1

06504

−34.4 (−23.5, − 50.4)

 

FLF1

12826

−2.9 (−2.8, − 3.0)

 

TFS1

06118

−2.6 (−2.4, − 2.8)

 

AGD1

14136

−22.1 (−20.5, − 24.0)

 
  1. aFusarium Comparative Database (Broadinstitute.org).
  2. bExpression was measured by quantitative reverse-transcriptase polymerase chain reaction (qPCR). RNA from biological replicate samples of WT or Δfst1 was pooled for cDNA synthesis, and three technical replicates were analyzed for each gene. Expression of TUB1 (FVEG_04081) was used to normalize data. For each gene, values represent fold differences in Δfst1 with WT expression set at a value of 1. Expression of each gene was calculated as 2ΔΔCt. Range of expression is in parentheses equals 2 ΔΔCt-s, 2 ΔΔCt+s, where s equals the standard deviation of the 2ΔΔCt value.
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BMC Microbiology

ISSN: 1471-2180

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