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Figure 1 | BMC Microbiology

Figure 1

From: Characterization of motility and piliation in pathogenic Neisseria

Figure 1

Average motility speed of Neisseria strains on a solid surface. (A) N. gonorrhoeae strains FA1090 and MS11, a nonpiliated MS11 and four clones of MS11 with different pilE sequences (i.e., 3:1, 5:1, 6:1, and 8:1). N. meningitidis strains FAM20 (serogroup C), C462 (serogroup W), C480 (serogroup C), JB515 (serogroup W), C311 (serogroup B) and the pilE sequence swap mutant in FAM20. The bacteria were allowed to adhere to poly-D-lysine-coated glass for 60 min at 37°C in 5% CO2-saturated GC broth. Attempts to track strains of serogroup A failed, as these strains did not adhere to the poly-D-lysine-coated surface. (B) Motility speed of N. gonorrhoeae FA1090 and N. meningitidis FAM20 on collagen-coated glass. The bacteria were allowed to adhere to the glass for 60 min prior to tracking. Motile bacteria were studied using live-cell imaging and particle tracking. The data are presented as the average values of at least 46 tracks acquired in at least three independent experiments. The error bars indicate the standard error of the mean. Significant differences (p < 0.05) between gonococcal and meningococcal strains are marked with an asterisk *.

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