Starvation survival assay for E. coli DH5α strains in PBS buffer for 15 days. For each strain, two independent replicates were performed. Each replicate includes. four repeats. Viable cells of the six strains drop sharply for the first six days. E. coli DH5α survived better, especially at day 3, than E. coli DH5α ΔglgB from day 0 to 9. The other four strains behaved similar with no obvious difference. After day 6, cells died at a very slow rate. At day 15, the number of colony-forming units (CFU) for the six E. coli DH5α strains converged together.