Figure 4From: Development of a serological assay to predict antibody bactericidal activity against non-typeable Haemophilus influenzae NTHi SBA protocol. Bacteria are diluted in Dulbecco’s PBS containing 1% (w/v) BSA and 0.1% (w/v) glucose (A); they are then incubated with baby rabbit complement (B), and serially diluted sera raised against the selected antigens (C). The microtiter plate layout for the NTHi SBA assay is shown in panel (D).Back to article page