Figure 7

Glucose effect on expression of a transcription reporter fusion of acsA. A - Wild type carrying an acsA::luxAB transcription fusion was inoculated in AB medium supplemented with 3 μg/ml tetracycline and 22 mM glucose was added at the indicated time point (vertical arrows); B -Effect of a cya mutation on expression of a transcription reporter fusion of acsA. Wild type and Δcya mutant carrying an acsA::luxAB transcription fusion were inoculated in AB medium supplemented with 3 μg/ml tetracycline and grown up to OD₅₉₅ = 1.0; C - ΔacsR mutant carrying an acsA::luxAB transcription fusion were inoculated in AB medium supplemented with 3 μg/ml tetracycline and 22 mM glucose was added at the indicated time point (vertical arrows). Luminescence produced by each strain harvested at the exponential phase was expressed by specific luciferase activities [relative light units (RLU) per biomass (OD₅₉₅)] as described in Figure 3.