Neutral lipid content in lipid droplets in HHV8-infected HUVEC cells. HUVEC cells were infected with HHV8 as described in Figure 1. 24 h before the indicated times, cells were seeded at a density of 2.0 × 105 in 35 mm glass-bottomed dishes and cultured at 37°C in a 5% CO2 incubator in a growth medium. On days 3, 14 and 24 post infection, cells were fixed and stained with Nile Red (for details see Methods), reported as green/yellow dots in the figure (panel A). The bar in the figure is 30 μm. Panel B represents the quantitative analysis of Nile Red green fluorescence intensity. At least 200 cells were individually selected and analyzed for each experimental group. Normalized data represent the percentage of the mean density value (intensity per pixel) ± standard error (SE). Significance was set up when p < 0.05 (*) or p < 0.01(**) vs. respective control (ANOVA and Fischer’s LSD as post hoc test).