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Table 2 Relative expression of genes involved in osteogenic differentiation

From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

  Day 3 Day 7
  Relative expression Fold difference Relative expression Fold difference
ALP     
Differentiation control 13.11 ± 0.34 7.51 7.69 ± 0.51 14.55
S. aureus UAMS-1 3.23 ± 0.25 1.85 0.27 ± 0.38 0.52
P. aeruginosa SAMMC-418 0.53 ± 0.35 0.30 0.07 ± 0.45 0.13
RUNX2     
Differentiation control 0.53 ± 0.82 3.54 0.91 ± 0.72 2.59
S. aureus UAMS-1 0.21 ± 0.99 1.42 1.24 ± 0.70 3.56
P. aeruginosa SAMMC-418 0.13 ± 0.62 0.85 1.35 ± 0.67 3.86
OCN     
Differentiation control 0.34 ± 0.36 1.92 31.91 ± 0.53 10.42
S. aureus UAMS-1 0.01 ± 0.35 0.08 0.01 ± 0.86 0.00
P. aeruginosa SAMMC-418 0.02 ± 0.27 0.12 0.00 ± 0.52 0.00
  1. ALP – alkaline phosphatase; RUNX2 – Runt-related transcription factor 2; OCN – bone gamma-carboxyglutamic acid-containing protein (osteocalcin).
  2. Values represent the mean ± SD relative expression of genes as determined by the 2-ΔCt method. Fold difference is the expression of normalized values as a ratio to undifferentiated growth controls.