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Table 1 Relative expression of genes involved in adipogenic differentiation

From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

 

Day 3

Day 7

 

Relative expression

Fold difference

Relative expression

Fold difference

PPAR γ

    

  Differentiation control

0.70 ± 0.37

14.45

0.11 ± 0.71

4.72

  S. aureus UAMS-1

0.29 ± 0.26

6.02

0.19 ± 0.50

8.19

  P. aeruginosa SAMMC-418

0.50 ± 0.29

10.31

0.20 ± 0.74

8.53

Adiponectin

    

  Differentiation control

0.00 ± 1.42

0.96

0.24 ± 1.12

376.63

  S. aureus UAMS-1

0.10 ± 1.35

147.34

0.01 ± 1.03

21.30

  P. aeruginosa SAMMC-418

0.00 ± 1.36

0.98

0.00 ± 1.60

2.77

Leptin

    

  Differentiation control

0.06 ± 1.02

0.22

0.90 ± 0.88

93.90

  S. aureus UAMS-1

0.01 ± 1.01

0.05

0.03 ± 0.60

2.61

  P. aeruginosa SAMMC-418

0.01 ± 1.00

0.04

0.00 ± 0.61

0.23

  1. PPARγ – peroxisome proliferator-activated receptor gamma.
  2. Values represent the mean ± SD relative expression of genes as determined by the 2-ΔCt method. Fold difference is the expression of normalized values as a ratio to undifferentiated growth controls.