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Figure 4 | BMC Microbiology

Figure 4

From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

Figure 4

Tube formation and migratory function of hBMSCs are differentially affected by the presence of soluble factors of biofilms. A) Representative light microscopy images of hBMSCs after 2, 6 and 16 h of angiogenic differentiation on gel matrices (40x magnification) in the presence of BCM (25%) from S. aureus and P. aeruginosa. B) Quantification of capillary-like tube number and C) tube length (μm) between connectors using Image J software (n = 6 images per group).* p < 0.05 compared to growth control at each timepoint. D) Representative fluorescent microscopy images of hBMSCs stained with Vybrant® CFDA SE Cell Tracer dye (green, 40x magnification) after 3 days of migration into void area in the presence of BCM (25%) from S. aureus and P. aeruginosa. E) Quantification of cell migration into void area based on measurement of fluorescence and image thresholding, using Image J software; *p < 0.05 compared to growth control.

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BMC Microbiology

ISSN: 1471-2180

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