Figure 1From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion Soluble factors from biofilms reduce viability in hBMSCs. Enumeration of viable bacteria within biofilms (A) and measurement of released protein from biofilms (B) of S. aureus UAMS-1 and P. aeruginosa SAMMC-418 grown for up to 5 days. (C) Cell viability of hBMSCs over a 7 day period following exposure to media containing 25% PBS (vehicle control), lipoteichoic acid (LTA, 10 μg/mL at 25%, Gram-positive cell wall component), S. aureus UAMS-1 planktonic-conditioned media (PCM), and S. aureus UAMS-1 biofilm-conditioned media (BCM). (D) Cell viability of hBMSCs over a 7 day period following exposure to media containing 25% PBS (vehicle control), lipopolysaccharides (LPS, 10 μg/mL at 25%, Gram-negative cell wall component), P. aeruginosa SAMMC-418 PCM, and P. aeruginosa SAMMC-418 BCM. Values are expressed as a ratio of the untreated growth control (untreated hBMSCs exposed to complete growth media, indicated by dashed lines) and represent the average ± SEM. *,#,+ p < 0.05 compared to day 1, 3 and 7 controls, respectively.Back to article page