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Table 2 Oligonucleotides used in this study

From: Characterization of the β-barrel assembly machine accessory lipoproteins from Borrelia burgdorferi

Name

Sequence (5’ to 3’, restriction sites in bold)

Description

BamA P1 (NheI) F

GCGGCTAGCAAGGGGAAAATAATAAAGGGTAT

bamA nucleotides 82–104 plus NheI site (N-terminus of POTRA 1)

BamA P4 + 14 (XhoI) R

GCGCTCGAGATTTTTATTTTTAGAAACAGTAATAG

Complementary to bamA nucleotides 1079–1104 plus XhoI site (14 aa into POTRA 5 domain)

bb0028 (NheI) F

GCGGCTAGCCTGCAAAAAATAAAACATGAATAC

bb0028 nucleotides 109–132 plus NheI site

bb0028 (XhoI) R

GCGCTCGAGTTCTTTAGTTAATTTTCTGTTTTCC

Complementary to bb0028 nucleotides 1023–1047 plus XhoI site

795a (BamHI) F

GCGGGATCCATGGGTTCAATTAGAGGTTTGT

bamA nucleotides 1–22 plus BamHI site

795a (XbaI) R

GCGTCTAGAATCAGGAACTTCCCTCTTGC

Complementary to bamA nucleotides 1562–1581 plus XbaI site

795b (SalI) F

GCGGTCGACCCATTTACAAGTTGGGAAGAAT

bamA nucleotides 1582–1593 plus SalI site

795b (PstI) R

GCGCTGCAGTCAATATCTCATCTCAATTCCTA

Complementary to bamA nucleotides 1562–1581 plus XbaI site

flgB (BamHI) F

GCGGGATCCTACCCGAGCTTCAAGGAAGAT

flgB promoter nucleotides 1–21 plus BamHI site

flgB (BamHI) R

GCGGGATCCATGGAAACCTCCCTCATTTAAA

Complementary to flgB nucleotides 387–408 plus BamHI site

c-Myc (XbaI-SalI) F

GCGTCTAGAGAACAAAAACTTATTTCTGAAGAAGATCTGGTCGACGCG

c-Myc tag plus 5’ XbaI and 3’ SalI sites

c-Myc (XbaI-SalI) RC

GCGGTCGACCAGATCTTCTTCAGAAATAAGTTTTTGTTCTCTAGACGC

Complementary to c-Myc tag plus 5’ SalI and 3’ XbaI sites

KO324 downstream (KpnI) F

GCGGGTACCGATTATTTGGGCAGATATCAAG

Complementary to nucleotides 330,248-330,269 of B31 chromosome (600 bp downstream of bb0324) plus KpnI site

KO324 downstream (XhoI) R

GCGCTCGAGTAATTTAAGAAATAAAAATTTTTACTG

Nucleotides 329,622-329,648 of B31 chromosome (immediately downstream of bb0324) plus XhoI site

KO324 upstream (BamHI) F

GCGGGATCCTTATAGCAATAATAAGCTTATAAAG

Nucleotides 328,661-328,685 of B31 chromosome (600 bp upstream of bb0324) plus BamHI site

KO324 upstream (EcoRI) R

GCGGAATTCTTCTGCCTCTTTTAGAATGTTTT

Complementary to nucleotides 329,239-329,262 of B31 chromosome (immediately upstream of bb0324), plus EcoRI site

flgB (XhoI) F

GCGCTCGAGTACCCGAGCTTCAAGGAAG

flgB nucleotides 1–21 plus XhoI site

Strep (EcoRI) R

GCGGAATTCTTATTTGCCGACTACCTTGGTGAT

Complementary to aadA nucleotides 769–792 plus EcoRI site

0028 F 4–29 (EcoRI&NdeI)

GCGGAATTCCATATGAAACAAAAATACGAAAACTATTTTAA

bb0028 nucleotides 4–29 plus EcoRI and NdeI sites

0028 R 678–699 (BamHI)

GCGGGATCCACCACCAGTCATTACTAAAACT

Complementary to bb0028 nucleotides 678–699 plus BamHI site

0027 F 4–29 (KpnI)

GCGGGTACCAGAAAGTATATTTTTATAATACTAAT

bb0027 nucleotides 4–29 plus KpnI site

0027 R 613–636 (XhoI)

GCGCTCGAGCAATTTATTTACATTCACTGTAAC

Complementary to bb0027 nucleotides 613–636 plus XhoI site