From: Characterization of the β-barrel assembly machine accessory lipoproteins from Borrelia burgdorferi
Name | Sequence (5’ to 3’, restriction sites in bold) | Description |
---|---|---|
BamA P1 (NheI) F | GCGGCTAGCAAGGGGAAAATAATAAAGGGTAT | bamA nucleotides 82–104 plus NheI site (N-terminus of POTRA 1) |
BamA P4 + 14 (XhoI) R | GCGCTCGAGATTTTTATTTTTAGAAACAGTAATAG | Complementary to bamA nucleotides 1079–1104 plus XhoI site (14 aa into POTRA 5 domain) |
bb0028 (NheI) F | GCGGCTAGCCTGCAAAAAATAAAACATGAATAC | bb0028 nucleotides 109–132 plus NheI site |
bb0028 (XhoI) R | GCGCTCGAGTTCTTTAGTTAATTTTCTGTTTTCC | Complementary to bb0028 nucleotides 1023–1047 plus XhoI site |
795a (BamHI) F | GCGGGATCCATGGGTTCAATTAGAGGTTTGT | bamA nucleotides 1–22 plus BamHI site |
795a (XbaI) R | GCGTCTAGAATCAGGAACTTCCCTCTTGC | Complementary to bamA nucleotides 1562–1581 plus XbaI site |
795b (SalI) F | GCGGTCGACCCATTTACAAGTTGGGAAGAAT | bamA nucleotides 1582–1593 plus SalI site |
795b (PstI) R | GCGCTGCAGTCAATATCTCATCTCAATTCCTA | Complementary to bamA nucleotides 1562–1581 plus XbaI site |
flgB (BamHI) F | GCGGGATCCTACCCGAGCTTCAAGGAAGAT | flgB promoter nucleotides 1–21 plus BamHI site |
flgB (BamHI) R | GCGGGATCCATGGAAACCTCCCTCATTTAAA | Complementary to flgB nucleotides 387–408 plus BamHI site |
c-Myc (XbaI-SalI) F | GCGTCTAGAGAACAAAAACTTATTTCTGAAGAAGATCTGGTCGACGCG | c-Myc tag plus 5’ XbaI and 3’ SalI sites |
c-Myc (XbaI-SalI) RC | GCGGTCGACCAGATCTTCTTCAGAAATAAGTTTTTGTTCTCTAGACGC | Complementary to c-Myc tag plus 5’ SalI and 3’ XbaI sites |
KO324 downstream (KpnI) F | GCGGGTACCGATTATTTGGGCAGATATCAAG | Complementary to nucleotides 330,248-330,269 of B31 chromosome (600 bp downstream of bb0324) plus KpnI site |
KO324 downstream (XhoI) R | GCGCTCGAGTAATTTAAGAAATAAAAATTTTTACTG | Nucleotides 329,622-329,648 of B31 chromosome (immediately downstream of bb0324) plus XhoI site |
KO324 upstream (BamHI) F | GCGGGATCCTTATAGCAATAATAAGCTTATAAAG | Nucleotides 328,661-328,685 of B31 chromosome (600 bp upstream of bb0324) plus BamHI site |
KO324 upstream (EcoRI) R | GCGGAATTCTTCTGCCTCTTTTAGAATGTTTT | Complementary to nucleotides 329,239-329,262 of B31 chromosome (immediately upstream of bb0324), plus EcoRI site |
flgB (XhoI) F | GCGCTCGAGTACCCGAGCTTCAAGGAAG | flgB nucleotides 1–21 plus XhoI site |
Strep (EcoRI) R | GCGGAATTCTTATTTGCCGACTACCTTGGTGAT | Complementary to aadA nucleotides 769–792 plus EcoRI site |
0028 F 4–29 (EcoRI&NdeI) | GCGGAATTCCATATGAAACAAAAATACGAAAACTATTTTAA | bb0028 nucleotides 4–29 plus EcoRI and NdeI sites |
0028 R 678–699 (BamHI) | GCGGGATCCACCACCAGTCATTACTAAAACT | Complementary to bb0028 nucleotides 678–699 plus BamHI site |
0027 F 4–29 (KpnI) | GCGGGTACCAGAAAGTATATTTTTATAATACTAAT | bb0027 nucleotides 4–29 plus KpnI site |
0027 R 613–636 (XhoI) | GCGCTCGAGCAATTTATTTACATTCACTGTAAC | Complementary to bb0027 nucleotides 613–636 plus XhoI site |