Table 2 Plasmids used in this study
From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
Plasmid | Relevant genotype | Source |
|---|---|---|
pPL82 | amyE integration plasmid | [29] |
pQE60 | His6 protein purification plasmid | Qiagen |
pNW5 | degU (H 12 L) in pPL82 | This study |
pNW43 | degU in pQE60 | [18] |
pNW52 | degU (D 56 N) in pQE60 | This study |
pNW54 | degU (H 12 L) in pQE60 | This study |
pNW1057 | degU (H 12 L, D 56 N) in pPL82 | This study |
pNW1072 | degU (H 12 L, D 56 N) in pQE60 | This study |