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Table 2 Plasmids used in this study

From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate

Plasmid Relevant genotype Source
pPL82 amyE integration plasmid [29]
pQE60 His6 protein purification plasmid Qiagen
pNW5 degU (H 12 L) in pPL82 This study
pNW43 degU in pQE60 [18]
pNW52 degU (D 56 N) in pQE60 This study
pNW54 degU (H 12 L) in pQE60 This study
pNW1057 degU (H 12 L, D 56 N) in pPL82 This study
pNW1072 degU (H 12 L, D 56 N) in pQE60 This study