Table 1 Bacillus subtilis strains used in this study
From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
Strain | Relevant genotype a | Source b |
|---|---|---|
NCIB3610 | Bacillus subtilis Prototroph | B.G.S.C. |
168 | Bacillus subtilis trpC2 | B.G.S.C. |
JH642 | Bacillus subtilis trpC2 pheA1 | [50] |
TMP147 | 168 ackA::mls | [48] |
DS1677 | 3610 Δhag | D. Kearns |
QB136 | leuB8 trpC2 degU32-hy | [30] B.G.S.C |
QB4144 | 168 degU146 | [12] |
NRS1136 | JH642 degS::pDH64 (cml) | [18] |
NRS1183 | JH642 ΔdegSU::spc | [21] |
NRS1287 | JH642 amyE::P hyspank -degU-32-hy-lacI (spc) | [18] |
NRS1314 | 3610 degU::pBL204 (cml) | [18] |
NRS1325 | 3610 degU::pBL204 (cml) amyE::P hy-spank -degU32-hy-lacI (spc) | [18] |
NRS1326 | 3610 degU::pBL204 (cml) amyE::P hy-spank -degU-lacI (spc) | [18] |
NRS1327 | 3610 degU::pBL204 (cml) amyE::P hy-spank -degU146-lacI (spc) | [18] |
NRS1358 | 3610 degS::pDH64 (cml) | [18] |
NRS1433 | JH642 pgsB::pBL141 (spc) | [21] |
NRS1499 | 3610 ΔdegSU::spc | SPP1 NRS1183 → NCIB3610 |
NRS4419 | 3610 ΔdegSU::spc amyE::P hy-spank -degU32-hy-lacI (spc) | SPP1 NRS1287 → NRS1499 |
NRS4756 | 168 amyE::P spac -degU32hy-degU146-lacI (cml) | pNW1057 → 168 |
NRS4761 | 3610 ΔdegSU::spc amyE::P spac -degU32-hy-degU146-lacI (cml) | SPP1 NRS4756 → NRS1499 |
NRS4762 | 3610 degU::mls amyE::P spac - degU32-hy-degU146-lacI (cml) | SPP1 NRS4756 → DS1993 |
NRS4770 | 3610 ackA::mls | SPP1 TMP147 → NCIB3610 |
NRS4771 | 3610 ackA::mls degS::pDH64 (cml) | SPP1 TMP147 → NRS1358 |
NRS4819 | 3610 degS::cml ackA::mls pgsB::spc | SPP1 NRS1443 → NRS4771 |