Figure 1
From: An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
Production of γ-PGA by strains expressing DegU protein variants. (A) Colony morphology of 3610 (wild type strain NCIB3610), degS (NRS1358), degU (NRS1314), degU + P hyspank -degU (H12L)-lacI (NRS1325) and ΔdegSU + P hyspank -degU (H12L)-lacI (NRS4419) grown on LB agar plates in the absence (−) or presence (+) of 50 μM IPTG. (B) Colony morphology of 3610 (wild type strain), degU + P hy-spank -degU (H12L, D56N)-lacI (NRS4762) and ΔdegSU + P hy-spank -degU (H12L, D56N)-lacI (NRS4761) grown on LB agar plates in the absence (−) or presence (+) of 50 μM IPTG. Plates were photographed after 16 h incubation at 37°C.