Table 1 Salmonella vaccine strains and plasmids used in this study
Strain | Salmonella Serovar | Genotype/Phenotype a | Reference |
|---|---|---|---|
χ9558 | Typhimurium | ∆pmi-2426 ∆(gmd-fcl)-26 ∆Pfur81::TT araC PBAD fur ∆Pcrp527::TT araC PBAD crp ∆asdA27::TT araC PBAD c2 ∆araE25 ∆araBAD23 ∆relA198::araC PBAD lacI TT ∆sopB1925 ∆agfBAC811, RpoS+ | [39] |
χ9633 | Typhi ISP1820 | ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD fur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBAD lacI TT ∆araE25 ∆araBAD23 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS+ | [13] |
χ9639 | Typhi Ty2 | ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD fur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBAD lacI TT ∆araE25 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS− | [13] |
χ9640 | Typhi Ty2 | ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD fur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBAD lacI TT ∆araE25 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS+ | [13] |
χ3761 | Typhimurium | wild type | [40] |
χ3744 | Typhi | ISP1820 wild type | [41] |
χ3769 | Typhi | Ty2 rpoS | [42] |
χ8438 | Typhi | Ty2 RpoS+ | [43] |
Plasmid | Description b | ||
pWSK129 | pSC101 ori, Kanr | [44] | |
pYA3493 | pBR ori, Asd+ vector with bla SS-based periplasmic antigen secretion | [45] | |
pYA4088 | Encodes the α-helical region of PspA (aa 3-285) in pYA3493 | [46] |