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Table 1 Salmonella vaccine strains and plasmids used in this study

From: Use of Ensure® nutrition shakes as an alternative formulation method for live recombinant Attenuated Salmonella Typhi vaccines

Strain Salmonella Serovar Genotype/Phenotype a Reference
χ9558 Typhimurium pmi-2426 ∆(gmd-fcl)-26 ∆Pfur81::TT araC PBAD fur ∆Pcrp527::TT araC PBAD crpasdA27::TT araC PBAD c2araE25araBAD23relA198::araC PBAD lacI TT ∆sopB1925agfBAC811, RpoS+ [39]
χ9633 Typhi ISP1820 ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD furpmi-2426 ∆(gmd-fcl)-26relA198::araC PBAD lacI TT ∆araE25araBAD23tviABCDE10agfBAC811sopB1925asdA33, RpoS+ [13]
χ9639 Typhi Ty2 ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD furpmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBAD lacI TT ∆araE25tviABCDE10agfBAC811sopB1925asdA33, RpoS [13]
χ9640 Typhi Ty2 ∆Pcrp527::TT araC PBAD crp ∆Pfur81::TT araC PBAD furpmi-2426 ∆(gmd-fcl)-26relA198::araC PBAD lacI TT ∆araE25tviABCDE10agfBAC811sopB1925asdA33, RpoS+ [13]
χ3761 Typhimurium wild type [40]
χ3744 Typhi ISP1820 wild type [41]
χ3769 Typhi Ty2 rpoS [42]
χ8438 Typhi Ty2 RpoS+ [43]
Plasmid   Description b  
pWSK129   pSC101 ori, Kanr [44]
pYA3493   pBR ori, Asd+ vector with bla SS-based periplasmic antigen secretion [45]
pYA4088   Encodes the α-helical region of PspA (aa 3-285) in pYA3493 [46]
  1. aIn genotype descriptions, the subscripted number refers to a composite deletion and insertion of the indicated gene. P, promoter; TT, T4 ip III transcription terminator.
  2. b ori, replication of origin; SS, secretion signal; Kanr, kanamycin resistance.