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Figure 3 | BMC Microbiology

Figure 3

From: Lactobacillus paracasei CNCM I-4034 and its culture supernatant modulate Salmonella-induced inflammation in a novel transwell co-culture of human intestinal-like dendritic and Caco-2 cells

Figure 3

Measurement of anti-inflammatory cytokines and TNF-α in DCs through an epithelial layer after exposure to L. paracasei , Salmonella or a combination of the two. The production of IL-10 (Panel A), TNF-α (Panel B), TGF-β1 (Panel C) and TGF-β2 (Panel D) was measured. DCs and Caco-2 cells were incubated for 4 h with the live probiotic L. paracasei or its supernatant, Salmonella or both and further incubated for 20 h in medium containing antibiotics. The culture supernatants were collected from the lower chamber, and the cytokine levels were determined by immunoassay. The data shown are the mean and SEM of the values obtained in three different experiments. *, p<0.05 compared with controls; #, p<0.05 compared with S.typhi; N.D. indicates not detected.

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