Figure 2

Concentrations of ergotamine (A) and ergine (B) after incubation with Rhodococcus erythropolis strains or buffer. Reactions contained biomass of R. erythropolis strains at OD_600nm = 2.0, which was equivalent to 31% (MTHt3, DSM 20665, DSM 43188) to 80% (DSM 11397) of the maximum OD_600nm reached in TSB, in sodium phosphate buffer pH 7.0 with 5 mg/l ergotamine or ergine, and were incubated at 25°C with shaking. Strains are referenced in Table 1.