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Table 1 Bacterial strains and plasmids used in this study

From: Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1

Strain or plasmid

Relevant characteristic

Source or reference

Bacterial strains

  

  Escherichia coli

  

 JM109

Host for cloning; recA1. endAl, gyrA96, thi. hsdR17, supE44, relA1, λ-, ∆(lac-proAB), [F’, traD36, proAB, lacI q Z∆M15]

[39]

 JM109λpir

Host for cloning pSMV10; JM109 lysogenized with λpir

[40]

 WM6026

Donor strain for conjugation; lacI q, rrnB3, DElacZ4787, hsdR514, DE(araBAD)567, E(rhaBAD)568, rph-1, att-lambda::pAE12-del(oriR6K-cat::frt5), DE(endA)::frt, uidA(delMluI)::pir(wt), attHK::pJK1006-del1/2 (deloriR6K-cat::frt5, deltrfA::frt)

William Metcalf, University of Illinois

 BL21 (DE3)

F− ompT hsdR17(r B − m B + ) gal dcm(DE3) F−, ompT, hsdS B(rB − mB −), gal(λcI 857, ind1, Sam7, nin5, lacUV5-T7gene1), dcm(DE3)

Novagen

  Shewanella oneidensis

 MR-1

Wild type

ATCC [2]

 ∆crp

The crp gene (SO_0624) disrupted

This study

Plasmids

  

 pMElacZ

pME4510 derivative, lacZ Gmr

[41]

 pMEomcA-54

pMElacZ containing the region from −54 to +93 relative to TSP omcA

This study

 pMEomcA-104

pMElacZ containing the region from −104 to +93 relative to TSP omcA

This study

 pMEomcA-150

pMElacZ containing the region from −150 to +93 relative to TSP omcA

This study

 pMEmtrC-42

pMElacZ containing the region from −42 to +119 relative to TSP mtrC

This study

 pMEmtrC-100

pMElacZ containing the region from −100 to +119 relative to TSP mtrC

This study

 pMEmtrC-144

pMElacZ containing the region from −144 to +119 relative to TSP mtrC

This study

 pMEmtrC-203

pMElacZ containing the region from −203 to +119 relative to TSP mtrC

This study

 pSMV10

9.1 kb mobilizable suicide vector; oriR6K, mobRP4, sacB, Kmr, Gmr

Chad Saltikov, California Institute of Technology

 pSMV-0624

1.5 kb fusion PCR fragment containing ∆crp cloned into the SpeI site of pSMV10

This study

 pET-28(a)

Expression vector, T7 promoter

Novagen

 pET-crp

pET-28(a) containing N-ht-crp

This study