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Table 1 Bacterial strains and plasmids used in this study

From: Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1

Strain or plasmid Relevant characteristic Source or reference
Bacterial strains   
Escherichia coli   
 JM109 Host for cloning; recA1. endAl, gyrA96, thi. hsdR17, supE44, relA1, λ-, ∆(lac-proAB), [F’, traD36, proAB, lacI q Z∆M15] [39]
 JM109λpir Host for cloning pSMV10; JM109 lysogenized with λpir [40]
 WM6026 Donor strain for conjugation; lacI q, rrnB3, DElacZ4787, hsdR514, DE(araBAD)567, E(rhaBAD)568, rph-1, att-lambda::pAE12-del(oriR6K-cat::frt5), DE(endA)::frt, uidA(delMluI)::pir(wt), attHK::pJK1006-del1/2 (deloriR6K-cat::frt5, deltrfA::frt) William Metcalf, University of Illinois
 BL21 (DE3) F ompT hsdR17(r B m B + ) gal dcm(DE3) F, ompT, hsdS B(rB mB ), gal(λcI 857, ind1, Sam7, nin5, lacUV5-T7gene1), dcm(DE3) Novagen
Shewanella oneidensis
 MR-1 Wild type ATCC [2]
 ∆crp The crp gene (SO_0624) disrupted This study
Plasmids   
 pMElacZ pME4510 derivative, lacZ Gmr [41]
 pMEomcA-54 pMElacZ containing the region from −54 to +93 relative to TSP omcA This study
 pMEomcA-104 pMElacZ containing the region from −104 to +93 relative to TSP omcA This study
 pMEomcA-150 pMElacZ containing the region from −150 to +93 relative to TSP omcA This study
 pMEmtrC-42 pMElacZ containing the region from −42 to +119 relative to TSP mtrC This study
 pMEmtrC-100 pMElacZ containing the region from −100 to +119 relative to TSP mtrC This study
 pMEmtrC-144 pMElacZ containing the region from −144 to +119 relative to TSP mtrC This study
 pMEmtrC-203 pMElacZ containing the region from −203 to +119 relative to TSP mtrC This study
 pSMV10 9.1 kb mobilizable suicide vector; oriR6K, mobRP4, sacB, Kmr, Gmr Chad Saltikov, California Institute of Technology
 pSMV-0624 1.5 kb fusion PCR fragment containing ∆crp cloned into the SpeI site of pSMV10 This study
 pET-28(a) Expression vector, T7 promoter Novagen
 pET-crp pET-28(a) containing N-ht-crp This study