Strain or plasmid | Relevant characteristic | Source or reference |
---|---|---|
Bacterial strains | Â | Â |
  Escherichia coli |  |  |
 JM109 | Host for cloning; recA1. endAl, gyrA96, thi. hsdR17, supE44, relA1, λ-, ∆(lac-proAB), [F’, traD36, proAB, lacI q Z∆M15] | [39] |
 JM109λpir | Host for cloning pSMV10; JM109 lysogenized with λpir | [40] |
 WM6026 | Donor strain for conjugation; lacI q, rrnB3, DElacZ4787, hsdR514, DE(araBAD)567, E(rhaBAD)568, rph-1, att-lambda::pAE12-del(oriR6K-cat::frt5), DE(endA)::frt, uidA(delMluI)::pir(wt), attHK::pJK1006-del1/2 (deloriR6K-cat::frt5, deltrfA::frt) | William Metcalf, University of Illinois |
 BL21 (DE3) | F− ompT hsdR17(r B − m B + ) gal dcm(DE3) F−, ompT, hsdS B(rB − mB −), gal(λcI 857, ind1, Sam7, nin5, lacUV5-T7gene1), dcm(DE3) | Novagen |
  Shewanella oneidensis | ||
 MR-1 | Wild type | ATCC [2] |
 ∆crp | The crp gene (SO_0624) disrupted | This study |
Plasmids | Â | Â |
 pMElacZ | pME4510 derivative, lacZ Gmr | [41] |
 pMEomcA-54 | pMElacZ containing the region from −54 to +93 relative to TSP omcA | This study |
 pMEomcA-104 | pMElacZ containing the region from −104 to +93 relative to TSP omcA | This study |
 pMEomcA-150 | pMElacZ containing the region from −150 to +93 relative to TSP omcA | This study |
 pMEmtrC-42 | pMElacZ containing the region from −42 to +119 relative to TSP mtrC | This study |
 pMEmtrC-100 | pMElacZ containing the region from −100 to +119 relative to TSP mtrC | This study |
 pMEmtrC-144 | pMElacZ containing the region from −144 to +119 relative to TSP mtrC | This study |
 pMEmtrC-203 | pMElacZ containing the region from −203 to +119 relative to TSP mtrC | This study |
 pSMV10 | 9.1 kb mobilizable suicide vector; oriR6K, mobRP4, sacB, Kmr, Gmr | Chad Saltikov, California Institute of Technology |
 pSMV-0624 | 1.5 kb fusion PCR fragment containing ∆crp cloned into the SpeI site of pSMV10 | This study |
 pET-28(a) | Expression vector, T7 promoter | Novagen |
 pET-crp | pET-28(a) containing N-ht-crp | This study |