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Figure 6 | BMC Microbiology

Figure 6

From: Transcriptional mechanisms for differential expression of outer membrane cytochrome genes omcA and mtrC in Shewanella oneidensis MR-1

Figure 6

Binding of CRP to the upstream regions of omcA and mtrC by EMSA. (A) DNA fragments used as probes are indicated with bars. The positions of the 5′ and 3′ ends of the fragments relative to TSP omcA or TSP mtrC (+1) are shown. The open boxes indicate the consensus regions upstream of TSP omcA or TSP mtrC shown in Figure 2. (B) Binding of CRP to each probe in the presence (+) or absence (−) of cAMP. The probes were generated by PCR and labeled with Cy3 at the 5′ termini. The quantities of CRP are indicated above.

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