Figure 1

The organization and transcriptional units of the mtr genes. (A) Schematic illustration of the organization of the mtr genes. Solid arrows indicate the location and direction of the transcriptional promoters upstream of omcA (P _omcA ) and mtrC (P _mtrC ). The bidirectional arrows indicate the target regions of the RT-PCR analysis. The open boxes indicate the CRP-binding regions identified by the EMSA analysis. (B) RT-PCR analysis of the mtr genes. WT cells were grown anaerobically in LM containing 10 mM fumarate until the early stationary growth phase. The lane number corresponds to the target regions shown in panel A. The molecular sizes (kb) of the marker (lane M) are indicated to the left of the gel.