Figure 4

Effect of hcp1 and hcp2 mutations on flagellin secretion into the extracellular matrix and on motility. A: Protein fractions from the ECM of MFE01, MFE01Δhcp1 and MFE01Δhcp1+hcp1 were analysed by SDS-PAGE and Coomassie staining. Bands with approximate molecular mass ≈ 38 kDa, indicated by the arrows, were observed at the growth temperature of 28°C and were identified as flagellin proteins by MALDI-TOF (n = 3). Molecular weight markers are indicated. B: Swimming assay on LB in 0.3% agar at 28°C. MFE01, MFE01Δhcp2 and MFE01Δhcp1+hcp1 were motile but MFE01Δhcp1 and MFE01Δhcp1+ pPSV35 (empty plasmid used as control) were non motile (n = 3).