Hcp secretion and mucoid phenotype in
strains. A: Mucoidy was assessed on LB agar at 28°C. +: mucoid, −: non mucoid. B: Mucoidy at 28°C or 37°C of MFE01. +: mucoid, −: non mucoid. The images shown are representative of three assays. C: Concentrated supernatants of cultures in late exponential growth phase, grown at 28°C or 37°C, were analysed by SDS-PAGE (15% separation gel) and Coomassie staining. Bands (indicated by arrows) with an approximate molecular mass of 21 kDa were observed in MFE01 and MFE07 supernatants at 28°C. Mass spectrometry identified these major supernatant proteins as Hcp proteins.