Table 6 Genes differentially expressed in Lactobacillus ruminis ATCC 27782
From: Lactobacillus ruminis strains cluster according to their mammalian gut source
Primer pair | ATCC 27782 | Function | |||||||
|---|---|---|---|---|---|---|---|---|---|
ID | RNA-seq | RT-PCR | |||||||
Swimming vs. Stationary log2 fold change a | pval | Swarming vs. Stationary log2 fold change b | pval | Fold change | 2ΔΔCT fold change swimming vs. Stationary c | 2ΔΔCT fold change swarming vs. Stationary d | |||
MMOD 1 | LRC_00640 | −2.97 | * | −0.03 | >0.05 | 8.00 |  |  | hypothetical protein |
MMOD 2 | LRC_00780 | −5.54 | *** | −0.94 | >0.05 | 24.00 | 0.02 (0.02-0.02) | 7.09 (6.35-7.93) | DeoR family transcriptional regulator |
MMOD 3 | pfkB | −4.10 | ** | −1.54 | >0.05 | 6.00 | 0.01 (0.01-0.01) | 0.08 (0.06-0.10) | 1-phosphofructokinase |
MMOD 4 | LRC_00800 | −3.18 | * | −0.74 | >0.05 | 5.00 | 0.2 (0.19-0.21) | 0.19 (0.16-0.23) | PTS system fructose-specific |
MMOD 5 | LRC_03250 | 2.62 | >0.05 | −0.60 | >0.05 | 9.00 | 17.92 (17.39-18.46) | 0.22 (0.18-0.27) | hypothetical protein |
MMOD 6 | LRC_04370 | 0.96 | >0.05 | 1.00 | >0.05 | 1.03 | 1.06 (1.01-1.10) | 0.22 (0.18-0.26) | hypothetical_protein |
MMOD 9 | LRC_05780 | 4.04 | ** | 0.89 | >0.05 | 9.00 | 28.54 (28.01-29.08) | 0.27 (0.23-0.32) | hypothetical protein |
MMOD 10 | LRC_06170 | 0.83 | >0.05 | −1.16 | >0.05 | 3.99 | 0.74 (0.70-0.77) | 0.41 (0.33-0.50) | flagellin |
MMOD 11 | iD = LRC_04600 | 1.35 | >0.05 | 2.42 | >0.05 | 2.10 | 8.31 (7.94-8.70) | 0.62 (0.51-0.75) | hypothetical_protein |
MMOD 12 | fliC | 1.27 | >0.05 | 0.16 | >0.05 | 2.15 | 1.83 (1.83-1.83) | 0.44 (0.36-0.54) | flagellin |
MMOD 13 | LRC_15700 | 1.07 | >0.05 | 0.20 | >0.05 | 1.82 | 1.48 (1.41-1.55) | 0.31 (0.25-0.38) | flagellin |
MMOD 14 | LRC_18780 | 5.11 | ** | −0.48 | >0.05 | 48.00 | 94.03 (89.54-98.74) | 0.19 (0.16-0.23) | PTS system sucrose-specific transporter subunit IIABC |
MMOD 15 | LRC_16260 | 3.70 | * | 1.16 | >0.05 | 6.00 | 2.13 (2.06-2.21) | 0.05 (0.04-0.07) | hypothetical protein |