From: Lactobacillus ruminis strains cluster according to their mammalian gut source
Test | Conc./Variable | Human | Bovine | Porcine | Equine | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
L5 | S21 | S23 | S36 | S38 | 25644 | 27780 | 27781 | 27782 | DPC 6830 | DPC 6831 | DPC 6832 | DPC 6833 | DPC 6834 | DPC 6835 | DPC 6836 | ||
Resistance assays | |||||||||||||||||
Bile Salt exposure | 0.25% | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
0.50% | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
0.75% | - | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
1% | - | + | + | + | + | + | + | + | + | + | + | + | + | + | - | + | |
2% | - | - | - | + | + | + | - | - | - | - | + | + | + | + | - | - | |
5% | - | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | |
pH resistance | 5.5 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
4.5 | - | - | - | - | - | - | - | + | + | + | + | + | + | + | + | + | |
4 | - | - | - | - | - | - | - | + | - | + | + | + | + | + | + | + | |
3.5 | - | - | - | - | - | - | - | - | - | - | + | + | + | + | + | + | |
3 | - | - | - | - | - | - | - | - | - | - | - | + | + | + | + | + | |
Chloramphenicol sensitivity | ≥4 μg/ml | S | S | S | S | R | R | S | S | R | R | S | S | R | R | S | R |
Rifampicin sensitivity | ≥1 μg/ml | S | S | S | S | S | S | S | S | S | S | S | S | S | S | S | S |
Simulated gastric juice | Survival (%) | 54 | 53 | 86 | 36 | 60 | 63 | 44 | 50 | 45 | 66 | 67 | 78 | 85 | 82 | 71 | 83 |
Biochemical assays | |||||||||||||||||
OPNG (β-galactosidase) |  | + | + | + | + | + | + | + | + | - | + | + | - | - | - | - | - |
API-ZYM | Leucine arylamidase (EC. 3.4.11.1) | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
 | Valine arylamidase | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | + | ++ | ++ | +/− | + | ++ | ++ | ++ |
 | Cystine arylamidase (EC. 3.4.11.3) | + | +/− | +/− | - | +/− | + | + | +/− | - | - | +/− | - | - | - | +/− | - |
 | Acid phosphatase (EC. 3.1.3.2) | +/− | +/− | + | +/− | +/− | +/− | ++ | + | +/− | + | + | ++ | + | + | +/− | ++ |
 | Naphthol-AS-BI-phosphohydrolase | + | + | + | + | +/− | +/− | + | + | + | + | + | + | + | + | + | + |
 | α-galactosidase (EC. 3.2.1.22) | + | + | + | +/− | +/− | + | + | + | + | ++ | ++ | ++ | + | +/− | +/− | + |
 | β-galactosidase (EC. 3.2.1.23) | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | - | ++ | ++ | - | - | - | - | - |
 | β-glucuronidase (EC. 3.2.1.31) | +/− | - | - | +/− | - | +/− | - | +/− | +/− | - | +/− | - | - | - | - | - |
 | α-glucosidase (EC. 3.2.1.20) | +/− | - | - | - | - | + | +/− | +/− | + | ++ | + | + | - | - | - | + |
 | β-glucosidase (EC. 3.2.1.21) | +/− | + | + | +/− | +/− | + | - | - | + | - | - | - | +/− | - | - | - |
 | N-acetyl-β-glucosaminidase (EC. 3.2.1.52) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | α-mannosidase (EC. 3.2.1.24) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | α-fucosidase (EC. 3.2.1.51) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | α-chymotrypsin (EC. 3.4.21.1) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | Trypsin (EC. 3.4.21.4) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | Alkaline phosphatase (EC. 3.1.3.1) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | Esterase (C4) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | Esterase lipase (C8) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
 | Lipase (C14) | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
EPS production | Glucose-MRS | + | + | - | + | - | + | + | + | - | + | - | + | - | + | - | + |
 | Sucrose-MRS | + | + | - | + | - | - | + | + | - | + | + | + | - | + | + | - |
 | Lactose-MRS | + | + | - | + | - | - | + | + | - | + | + | - | - | - | - | - |