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Figure 3 | BMC Microbiology

Figure 3

From: Development of a Multiplex-PCR probe system for the proper identification of Klebsiella variicola

Figure 3

Multiplex-PCR amplification of oligonucleotides combinations used for identification of K. variicola . A) Lane 1, molecular weight 1 kb; Lane 2, oligonucleotide combination named M-PCR-1 (KV770-F/KV770-R, KP888-F/KP888-R and KmtnC-F/KmtnC-R); Lane 3, oligonucleotide combination named M-PCR-2 (KV1615-F/KV1615-R, KP878-F/KP878-R and KmtnC-F/KmtnC-R); Lane 4, oligonucleotide combination named M-PCR-3 (KV1000-F/KV1000-F, KP888-F/KP888-R and KmtnC-F/KmtnC-R) (Table 1). B) M-PCR-1 assayed on environmental endophytic K. variicola isolates. Lane 1, molecular weight ΦX174 DNA-Hae III; Lane 2, K. pneumoniae ATCC 13883 and K. variicola 801, DNA’s combination; Lane 3, K. variicola T29A; Lane 4, K. variicola 6A2; Lane 5, K. variicola CFNE 2006; Lane 6, K. variicola 3; Lane 7, K. variicola F2R9; Lane 8, K. variicola VI; Lane 9, negative control (without DNA).

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