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Table 1 Kinin-like peptides in the Sap-treated LK samples that were identified using LC-MS/MS

From: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

Sap Kinin-like peptide Molecular mass (Da)
Sap1 MKRPPGFSPFR 1318.8
  MKRP(POH)GFSPFR 1334.7
  (MO)KRPPGFSPFR 1334.7
  MKRPPGFSPF 1162.8
  RPPGFSPFR 1059.8
  PPGFSPFR 903.4
Sap2 MKRPPGFSPFR 1318.8
  MKRP(POH)GFSPFR 1334.7
  (MO)KRPPGFSPFR 1334.7
  RPPGFSPFR 1059.8
  LMKRPPGFSPFR 1431.9
  MKRPPGFSPF 1162.8
  RP(POH)GFSPF 919.7
Sap3 MKRPPGFSPFR 1318.7
  MKRP(POH)GFSPFR 1334.7
  (MO)KRPPGFSPFR 1334.7
  (MO)KRP(POH)PGFSPFR 1350.7
  RPPGFSPFR 1059.8
  LMKRPPGFSPFR 1432.0
  LMKRP(POH)GFSPFR 1447.8
  L(MO)KRPGFSPFR 1447.8
Sap4 MKRPPGFSPFR 1318.7
  MKRP(POH)GFSPFR 1334.7
  (MO)KRPPGFSPFR 1334.7
  (MO)KRP(POH)PGFSPFR 1350.7
  RPPGFSPFR 1059.8
  RP(POH)GFSPF 919.7
Sap5 MKRPPGFSPFR 1318.8
  RPPGFSPFR 1059.8
Sap6 MKRPPGFSPFR 1318.8
  RPPGFSPFR 1059.8
  MKRP(POH)GFSPFR 1334.7
Sap8 MKRPPGFSPFR 1318.7
  MKRP(POH)GFSPFR 1334.7
  (MO)KRPPGFSPFR 1334.7
  (MO)KRP(POH)PGFSPFR 1350.7
  RPPGFSPFR 1059.8
  MKRPPGFSPF 1162.8
Sap9 MKRPPGFSPFR 1318.9
  RPPGFSPFR 1059.6
  PPGFSPFR 903.5
  P(POH)GFSPFR 919.5
  LMKRPPGFSPFR 1432.0
  LMKRP(POH)GFSPFR 1447.8
  PPGFSPF 747.4
  1. LK samples (1.5 μM) were digested with 0.03 μM Sap in citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (at a final concentration of 10 μM), and the samples were acidified with HCl (0.33 M) and subjected to LC-MS/MS analysis using the Bruker HCTultra ETDII IT mass spectrometer that was equipped with an ESI ion source and ETD II fragmentation module and was coupled to the Dionex Ultimate 3000 UHPLC system. MS/MS data were analyzed using in-house Mascot server (version 2.3.0), and the peptides were identified by searching the Swiss-Prot database. The peptides marked in bold generated peaks with the highest intensities (> 105 arbitrary units), which exceeded by ≥ 1 order of magnitude in comparison with the other peptides.