Figure 7From: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans Time course of the cleavage of the MKRPPGFSPFRSSR peptide using Sap9. MKRPPGFSPFRSSR peptide (5 μM) was cleaved using 0.1 μM Sap9 in 50 mM citrate buffer (pH 5.0) at 37°C for the specified time. The reaction was stopped using HCl, and the sample was analyzed using HPLC as specified in Figure 1. The results from a representative kinetic experiment are shown. The areas under the peaks of separated peptides are expressed relative to the substrate at the beginning of the reaction.Back to article page