Analysis of Paracoccidioides secreted proteins reveals fructose 1,6-bisphosphate aldolase as a plasminogen-binding protein

Chaves, Edilânia Gomes Araújo; Weber, Simone Schneider; Báo, Sonia Nair; Pereira, Luiz Augusto; Bailão, Alexandre Melo; Borges, Clayton Luiz; Soares, Célia Maria de Almeida

doi:10.1186/s12866-015-0393-9

Table 1 Secreted proteins of Paracoccidioides that bind plasminogen

From: Analysis of Paracoccidioides secreted proteins reveals fructose 1,6-bisphosphate aldolase as a plasminogen-binding protein

Spot number ¹	General information number (NCBI) ²	Protein description	pI theor/exp ³	MM (kDa) theor/exp ⁴	PMF score ⁵	Coverage sequence (%) ⁶	MS/MS Ions score ⁷	Matched peptides ⁸	Psort prediction ⁹	SignalP Value ≥ 0.5 ¹⁰	SecretomeP Value ≥ 0.5 ¹¹	big-PI ¹²
1	gi\|226285916	aminomethyl transferase	9.67/4.42	53.35/45.56	121	37	84	4	mito: 23.0	NO	0.516	NO
2	gi\|226278634	aldehyde dehydrogenase	5.92/6.94	54.69/45.97	94	59	114	6	cyto: 21.5	NO	0.562	NO
3	gi\|295668479	formamidase	6.10/7.13	46.14/45.71	144	44	109	5	cyto: 12.0	NO	0.565	NO
4	gi\|295658700	homogentisate 1,2-dioxygenase	6.25/7.62	50.85/45.51	78	35	-	-	cyto: 13.0	NO	0.601	NO
5	gi\|295658700	homogentisate 1,2-dioxygenase	6.16/7.76	50.86/45.35	76	26	89	4	cyto: 13.0	NO	0.621	NO
6	gi\|295659664	NADP-specific glutamate dehydrogenase	7.66/8.48	50.38/45.35	102	53	72	4	cyto: 11.0	NO	NO	NO
7	gi\|295659664	NADP-specific glutamate dehydrogenase	7.17/8.75	50.46/45.45	101	56	117	2	cyto: 11.0	NO	NO	NO
8	gi\|295669690	phosphoglycerate kinase	6.48/9.49	45.31/ 44.54	83	61	151	5	cyto: 25.0	NO	NO	NO
9	gi\|295666179	2-methylcitrate synthase	9.02/9.73	51.51/45.00	-	-	226	6	mito: 27.0	NO	NO	NO
10	gi\|295666179	2-methylcitrate synthase	9.02/9.96	51.51/ 44.92	95	62	95	4	mito: 27.0	NO	NO	NO
11	gi\|295666179	2-methylcitrate synthase	9.02/10.66	51.58/47.76	78	57	88	4	mito: 27.0	NO	NO	NO
12	gi\|295658119	glyceraldehyde-3-phosphate dehydrogenase	10.18/10.67	33.92/43.65	-	-	93	2	cyto: 27.0	NO	0.532	NO
13	gi\|295671120	fructose 1,6-bisphosphate aldolase	6.09/6.41	39.72/41.29	-	-	154	5	cyto: 21.0	NO	0.505	NO
14	gi\|295671120	fructose 1,6-bisphosphate aldolase	6.09/6.60	39.72/41.29	-	-	670	5	cyto: 21.0	NO	0.505	NO
15	gi\|295671120	fructose 1,6-bisphosphate aldolase	6.09/6.88	39.72/40.94	-	-	555	9	cyto: 21.0	NO	0.505	NO
16	gi\|295669690	phosphoglycerate kinase	6.48/7.75	45.31/42.67	86	59	56	3	cyto: 25.0	NO	NO	NO
17	gi\|295668707	acetyl-CoA acetyltransferase	8.98/7.88	46.65/42.67	-	-	102	3	mito: 24.5	NO	0.692	NO
18	gi\|11496183	immunodominant antigen Gp43	7.17/8.15	45.77/42.42	97	43	102	4	extr: 24.0	NO	0.746	NO
19	gi\|226285552	ketol-acid reductoisomerase	9.12/8.46	44.86/42.17	172	62	134	7	mito: 27.0	NO	0.683	NO
20	gi\|295658218	malate dehydrogenase	6.36/7.18	34.67/33.98	73	47	69	5	cyto: 17.0	NO	0.674	NO
21	gi\|295658218	malate dehydrogenase	6.36/7.85	34.67/33.75	129	41	344	9	cyto: 17.0	NO	0.674	NO
22	gi\|226279168	2,5-diketo-D-gluconic acid reductase A	7.71/8.40	34.78/33.36	81	48	50	3	cyto: 20.5	0.5	NO	NO

¹Spots numbers indicated in Figure 1A.
²NCBI database general information number (http://www.ncbi.nlm.nih.gov/).
³Isoelectric point (theoretical/experimental).
⁴Molecular Mass in kDa (theoretical/experimental);
⁵Mascot PMF score for fragmentation data (http://www.matrixscience.com).
⁶Sequence coverage percentage.
⁷Mascot MS/MS score for fragmentation data (http://www.matrixscience.com).
⁸Number of identified peptides (MS/MS).
⁹Subcellular localization prediction of proteins according Psort (http://www.genscript.com/psort/wolf_psort.html).
¹⁰Secretion prediction according to Signal P 3.0 server. The number corresponds to signal peptide probability (Score³ 0.5) (http://www.cbs.dtu.dk/services/SignalP/).
¹¹Secretion prediction according to Secretome P 1.0 server; the number corresponds to neural network that exceeded a value of 0.5 (NN-score ³ 0.50) (http://www.cbs.dtu.dk/services/SecretomeP/).
¹²GPI Modification Site Prediction of proteins according big-PI (http://mendel.imp.ac.at/gpi/gpi_server.html).
cyto: cytoplasm.
extr: extracellular.
mito: mitochondria.

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ISSN: 1471-2180

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