Figure 5

Paracoccidioides adhesion/internalization by macrophage: Effects of plasminogen and FBA. (A) Western blotting analysis of binding of J774 macrophages and rFBA. Macrophages were incubated with 50 μg rFBA (line 2). Additionally controls were performed with 5 μg of rFBA for the positive control (line 3), and 5 μg of BSA for negative control (line 4). Line 1 depicts macrophages, without incubation with the rFBA. The immunoblot was probed with the polyclonal antibodies directed to the rFBA. (B) Macrophages were or not pre-incubated with the rFBA (50 μg) for 1 h, before infection. Yeast cells (Pb01) were or not treated with the antibodies to the rFBA (Ab, 1:1000 diluted), with hPlg (50 μg) and tPA (50 ng), for 1 h, or with the antibodies to the rFBA (Ab, 1:1000 diluted) and subsequently with Plg (50 μg), and tPA (50 ng), for 1 h. Macrophages were incubated with the yeast cells above for 12 h at 36°C. * : results significantly different from control, at a p value < 0.05.