Figure 1From: Analysis of Paracoccidioides secreted proteins reveals fructose 1,6-bisphosphate aldolase as a plasminogen-binding protein Detection of plasminogen-binding proteins of Paracoccidioides , Pb 01. Secreted proteins (500 μg) of Paracoccidioides, Pb01, were subjected to 2-DE [first dimension: IEF pH range 3–11 non-linear, second dimension: 12% (w/v) SDS-PAGE)]. 2-DE gels were stained using Coomassie brilliant blue (Panel A) or transferred to nitrocellulose membranes that were processed for Far-western blotting (Panel B). Negative controls include a membrane not incubated with Plg (Panel C). Membrane in panel B was incubated with 35 μg/ml of hPlg, followed by the incubation with the anti-hPlg antibody (1 μg/mL) and the reaction was developed with BCIP/NBT. To identify in the 2-DE gel, the proteins spots that were visualized in the membrane, a pairing of proteins spots was performed, using Image master 2D Platinum software (GE Healthcare). The pH gradient is shown above the gel, and the molecular mass protein standards (kDa) are indicated to the left of the gels.Back to article page