Figure 2
From: Extracellular ATP inhibits twitching motility-mediated biofilm expansion by Pseudomonas aeruginosa
Extracellular ATP decreases levels of surface-assembled tfp. (A) ELISA of surface assembled tfp on whole cells from P. aeruginosa strains PAK and PAKpilA obtained from overnight (20 h) confluent lawns grown at 37°C on LB agar plates containing 0 mM or 10 mM ATP. Levels of surface assembled tfp were detected with anti-PilA anti-serum. Data are presented as the mean ± SD and are representative of results obtained from four independent experiments run in triplicate. (B) Immunoblot of pilin (PilA) detected in whole cell (WC) preparations (top panel) and in sheared surface tfp preparations (bottom panel) of strains PAK (lanes 1 – 4) and PAKpilA (lane 5) obtained from overnight (20 h) confluent lawns grown at 37°C on LB agar plates containing 0 mM or 10 mM ATP. Equal amounts of cells were used in each assay. The results shown in the immunoblots are representative of results obtained in four independent experiments run in duplicate.