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Table 2 Purification and yield of rMP1102

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

  Volume (ml) Protein concentration (mg/l) Specific activity (AU *10000/ml) Total activity (AU*100000) Recovery (%)
Culture supernatant 1000 1213.64 ± 42.68 15.36 ± 0.43 1536 100
Sephadex G-25 2600 357.31 ± 17.14 5.37 ± 0.08 1397 90.95
SP FF
Sepharose 820 376.89 ± 15.83 15.52 ± 0.36 1273 82.93