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Table 2 Purification and yield of rMP1102

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

 

Volume (ml)

Protein concentration (mg/l)

Specific activity (AU *10000/ml)

Total activity (AU*100000)

Recovery (%)

Culture supernatant

1000

1213.64 ± 42.68

15.36 ± 0.43

1536

100

Sephadex G-25

2600

357.31 ± 17.14

5.37 ± 0.08

1397

90.95

SP FF

Sepharose

820

376.89 ± 15.83

15.52 ± 0.36

1273

82.93