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Table 1 Productivity related parameters of Pichia pastoris GAPMP1102 for fed-batch fermentation under different pH conditions

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

  pH 5.0 pH 5.5 pH 6.0 pH 6.5 pH 7.0
Total protein (mg/l) 807.42 ± 26.35 913.28 ± 22.94 1076.99 ± 35.86 1213.64 ± 42.68 1127.38 ± 37.62
rMP1102 (mg/l) 367.59 ± 13.51 423.62 ± 18.27 486.35 ± 17.96 538.17 ± 21.32 503.92 ± 19.85
Activity (AU/ml × 10000) 3.84 ± 0.31 5.12 ± 0.28 10.24 ± 0.36 15.36 ± 0.43 10.24 ± 0.37
Max cell wet weight (g/l) 341.2 ± 19.41 378.5 ± 17.13 410.7 ± 16.29 424.9 ± 25.81 402.6 ± 21.74
Specific cell growth rate (μ) 0.188 0.189 0.208 0.224 0.198
Productivity (AU/mg rMP1102) 104464 120863 210548 285412 203207