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Figure 1 | BMC Microbiology

Figure 1

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

Figure 1

Construction of the recombinant expression vector pGAPMP1102. α-factor: native S. cerevisiae α-mating factor secretion signal, which can be self-cleaved by the endogenous Kex2 protease to leave a native sequence of target peptide; pAOX: methanol-inducible alcohol oxidase one promoter from P. pastoris; pGAP: glyceraldehyde-3-phosphate dehydrogenase promoter from P. pastoris; PTEF1: transcription elongation factor one gene promoter; PEM7: synthetic prokaryotic promoter; Zeocin: Zeocin resistance gene; pUC ori: replication and maintenance of the plasmid in E. coli; CYC1 TT: transcription termination region.

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BMC Microbiology

ISSN: 1471-2180

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