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Table 2 Bacterial strains and plasmids used in this study

From: The VieB auxiliary protein negatively regulates the VieSA signal transduction system in Vibrio cholerae

Strain Description Protein expressed Reference/Source
AC50 V. cholerae classical O395 biotype N/A 4
AC4713 E. coli BL21 (DE3) pGEX-TEV::vieS-C GST-VieS-C This study
AC4714 E. coli BL21 (DE3) pMMB67eh::vieA-His 6 VieA-His6 32
AC4715 E. coli BL21 (DE3) pET15b-TEV::His6-vieB His6-VieB This study
AC4716 E. coli BL21 (DE3) pET15b-TEV::His6-vieB D62A His6-VieB D62A This study
AC4717 E. coli BL21 (DE3) pET15b-TEV::His6-vieB D62E His6-VieB D62E This study
AC4002 E. coli BL21 (DE3) pMalc2E-TEV::vieS-C MBP-VieS D1018A 13