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Table 2 Bacterial strains and plasmids used in this study

From: The VieB auxiliary protein negatively regulates the VieSA signal transduction system in Vibrio cholerae

Strain

Description

Protein expressed

Reference/Source

AC50

V. cholerae classical O395 biotype

N/A

4

AC4713

E. coli BL21 (DE3) pGEX-TEV::vieS-C

GST-VieS-C

This study

AC4714

E. coli BL21 (DE3) pMMB67eh::vieA-His 6

VieA-His6

32

AC4715

E. coli BL21 (DE3) pET15b-TEV::His6-vieB

His6-VieB

This study

AC4716

E. coli BL21 (DE3) pET15b-TEV::His6-vieB D62A

His6-VieB D62A

This study

AC4717

E. coli BL21 (DE3) pET15b-TEV::His6-vieB D62E

His6-VieB D62E

This study

AC4002

E. coli BL21 (DE3) pMalc2E-TEV::vieS-C

MBP-VieS D1018A

13

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BMC Microbiology

ISSN: 1471-2180

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