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Table 2 Variable antimicrobial drug susceptibility testing by broth microdilution susceptibility testing with Merlin Micronaut system (μg/ml), biochemical characteristics, API Coryne and Vitek2-compact profiles of 10 Corynebacterium ulcerans field isolates from water rats

From: Outbreak with clonally related isolates of Corynebacterium ulcerans in a group of water rats

isolate ID TET TILM D-Trehalose API Coryne profile; Vitek2 CBC biotype number Vitek2 ANC biotype number
(interpretation/ % ID) (interpretation/ % ID) (interpretation/ % ID)
131010012-1 R (>16) R (32) - 0 111 326 (Cul/99.7) 15431340407010 (Cul/97.0) 2363060410505 (Cul/99.0)
131010012-2 R (>16) R (32) + 0 111 326 (Cul/99.7) 15431340447010 (Cul/95.0) 2363020410505 (Cul/99.0)
131011719 R (>16) R (32) + 0 111 326 (Cul/99.7) 15031340406010 (Cul/99.0) 2363021414505 (Cul/91.0)
131013415-1 S (≤1) R (32) + 0 111 326 (Cul/99.7) 15431340447010 (Cul/94.0) 2363020400505 (Cul/97.0)
131013415-2 S (≤1) R (32) - 0 111 326 (Cul/99.7) 15030340407010 (Cul/97.0) 2363020410505 (Cul/99.0)
131015432-10 S (≤1) R (32) (+) 0 111 326 (Cul/99.7) 15431340447010 (Cul/94.0) 2363060410505 (Cul/95.0)
131015432-13 S (≤1) I (16) + 0 111 366 (Cul/99.9) 15431340447010 (Cul/94.0) 2363020410505 (Cul/99.0)
131015432-14 S (≤1) I (16) - 0 111 326 (Cul/99.7) 15431340407010 (Cul/97.0) 2363021410505 (Cul/95.0)
141001018 S (≤1) I (16) + 0 111 767 (Cul/99.9) 15431340407010 (Cul/98.0) 2363060412505 (Cul/90.0)
141001548 R (16) I (16) + 1 111 326 (Cul/98.0) 15431340407010 (Cul/97.0) 2363020410505 (Cul/99.0)
  1. Susceptibility was determined by using the Clinical Laboratory Standards Institute criteria for broth microdilution susceptibility testing for Corynebacterium spp [50].
  2. TET tetracycline,TILM tilmicosin, R resistant, I intermediate susceptible, S susceptible phenotype + positive, negative, (+) weak reaction, Cul C. ulcerans.