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Table 4 Plasmids used in this study

From: N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase

Plasmid

Description

Source

pRS426

2 μ replication origin, URA3 marker

[34]

pYPS1

pRS426 expressing the YPS1 gene from its own promoter

[8]

pYPS1-C2

pYPS1 with D101E mutation

[8]

pYPS1-DL

pYPS1 in which the Yps1p N-ent loop is replaced by the Yps3p codons 98 to 102

This study

pYPS1-DL2

pYPS1-DL with D101E mutation

This study

pBA22H (pssYPS1)

2 μ replication origin, expressing a truncated version of Yps1p (Δ67 C-ter) with a 6×His-tag

[33]

pssYPS1-DL

pssYPS1 in which the Yps1 N-ent loop is replaced by the Yps3p codons 98 to 102

This study

pssYPS1-DL2

pssYPS1-DL with D101E mutation

This study