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Table 4 Plasmids used in this study

From: N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase

Plasmid Description Source
pRS426 2 μ replication origin, URA3 marker [34]
pYPS1 pRS426 expressing the YPS1 gene from its own promoter [8]
pYPS1-C2 pYPS1 with D101E mutation [8]
pYPS1-DL pYPS1 in which the Yps1p N-ent loop is replaced by the Yps3p codons 98 to 102 This study
pYPS1-DL2 pYPS1-DL with D101E mutation This study
pBA22H (pssYPS1) 2 μ replication origin, expressing a truncated version of Yps1p (Δ67 C-ter) with a 6×His-tag [33]
pssYPS1-DL pssYPS1 in which the Yps1 N-ent loop is replaced by the Yps3p codons 98 to 102 This study
pssYPS1-DL2 pssYPS1-DL with D101E mutation This study