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Table 3 Oligonucleotides used in this study

From: N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase

Name Description Sequence
YPS1 F Strain deletion confirmation 5′-CTTTTATACTGCGCCATGAGTAGTT-3′
PMT1 F Strain deletion confirmation 5′-CTTCAATTACGCTTTCTACCAACAT-3′
PMT2 F Strain deletion confirmation 5′-ACATATTATTGGACACGTCGCCC-3′
PMT4 F Strain deletion confirmation 5′-TTCCTACTTGTCCTTTCTTTCCTTT-3′
KANB Strain deletion confirmation 5′-CTGCAGCGAGGAGCCGTAAT-3′
Loop F N-ent loop replacement in pRSYPS1 5′-[PO4]-GCTCGGATAATCCATACTGTGGT TCTGTGATGGACTGTCAACAATACGG-3′
YB E101-F Mutation D101E 5′-GGTCCTGGTGGAAACAGGCTCCTCT-3′
YB E101-R Mutation D101E 5′-AGAGGAGCCTGTTTCCACCAGGACC-3′
YB40 Amplification of pYPS1-DL for gap repair in pssYPS1 5′-TGGAAGTGGGCACGCCAC-3′
YB43 Amplification of pYPS1-DL for gap repair in pssYPS1 5′-GAACACTATTTCCATACT-3′