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Table 1 N-terminal sequencing of purified Yps1 species

From: N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase

Sequenced sample Culture medium pH Molecular mass (kDa) Identified residues at each cycle* Deduced sequence
yps1∆ Yps1-DL 3.0 48 A1D2G3Y4E5E6I7I8I9T10N11Q12Q13S14F15 A68DGYEEIIITNQQSF
yps1∆ ssYps1-DL 6.0 48 A1D2G3Y4E5E6I7I8I9T10N11Q12Q13S14F15 A68DGYEEIIITNQQSF
yps1∆ ssYps1-DL 6.0 52 D1D2D3S4N5S6K7F8V9K10L11P12F13H14(K)15 D31DDSNSKFVKLPFHK
yps1∆kex2∆ ssYps1-DL 6.0 54 K1I2I3P4A5A6N7K8R9D10D11D12S13N14S15 K22IIPAANKRDDDSNS
  1. *:number in exponent matches the cycle number.