N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase

Table 1 N-terminal sequencing of purified Yps1 species

Sequenced sample	Culture medium pH	Molecular mass (kDa)	Identified residues at each cycle*	Deduced sequence
yps1∆ Yps1-DL	3.0	48	A¹D²G³Y⁴E⁵E⁶I⁷I⁸I⁹T¹⁰N¹¹Q¹²Q¹³S¹⁴F¹⁵	A⁶⁸DGYEEIIITNQQSF
yps1∆ ssYps1-DL	6.0	48	A¹D²G³Y⁴E⁵E⁶I⁷I⁸I⁹T¹⁰N¹¹Q¹²Q¹³S¹⁴F¹⁵	A⁶⁸DGYEEIIITNQQSF
yps1∆ ssYps1-DL	6.0	52	D¹D²D³S⁴N⁵S⁶K⁷F⁸V⁹K¹⁰L¹¹P¹²F¹³H¹⁴(K)¹⁵	D³¹DDSNSKFVKLPFHK
yps1∆kex2∆ ssYps1-DL	6.0	54	K¹I²I³P⁴A⁵A⁶N⁷K⁸R⁹D¹⁰D¹¹D¹²S¹³N¹⁴S¹⁵	K²²IIPAANKRDDDSNS

ISSN: 1471-2180