Figure 6From: N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase O -glycosylation of the Yps1 S/T-rich domain involves the Pmt2 O -mannosyltransferase. Yeast simple and double mutants transformed with Yps1 were grown 24 h at pH 3.0 and equal amounts of cell extracts treated with Endo Hf were analyzed by 10% SDS-PAGE and immunoblotted with an Yps1 antiserum recognizing both the β and α subunits (294–3).Back to article page